Validation Data Gallery
Tested Applications
| Positive WB detected in | Chicken egg |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-67405 targets Chicken IgY in WB applications and shows reactivity with Chicken samples.
| Tested Reactivity | Chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Chicken IgY |
| Observed molecular weight | 180 kDa, 70 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883846 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Chicken IgY antibody HRP-67405 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |