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  • Featured Product
  • KD/KO Validated

Chk1 Polyclonal antibody, PBS Only

Chk1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25887-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Polyclonal antibody (25887-1-AP)

    WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using Chk1 Polyclonal antibody (25887-1-AP)

    IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Chk1 Polyclonal antibody (25887-1-AP)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Chk1 Polyclonal antibody (25887-1-AP)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using Chk1 Polyclonal antibody (25887-1-AP)

    IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using Chk1 Polyclonal antibody (25887-1-AP)

    FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25887-1-PBS targets Chk1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chk1 fusion protein Ag22993 相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.