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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chk1 Recombinant antibody

Chk1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G7

Cat no : 80056-2-RR

Synonyms

CHEK1, CHK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Recombinant antibody (80056-2-RR)

    WB analysis using 80056-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80056-2-RR (Chk1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using Chk1 Recombinant antibody (80056-2-RR)

    IF Staining of HEK-293T using 80056-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using Chk1 antibody (80056-2-RR, Clone: 2G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80056-2-RR targets Chk1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for Chk1 antibody 80056-2-RRDownload protocol
IF protocol for Chk1 antibody 80056-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols