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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Chk1 Recombinant antibody

Chk1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G7

Cat no : CL488-80056-2

Synonyms

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Chk1 Recombinant ant (CL488-80056-2)

    IF Staining of HeLa using CL488-80056-2

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Chk1 antibody (CL488-80056-2, Clone: 2G7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated Chk1 Recombinant ant (CL488-80056-2)

    FC experiment of HEK-293T using CL488-80056-2

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Chk1 Recombinant antibody (CL488-80056-2, Clone:2G7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80056-2 targets Chk1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Chk1 antibody CL488-80056-2Download protocol
FC protocol for CL Plus 488 Chk1 antibody CL488-80056-2Download protocol
Standard Protocols
Click here to view our Standard Protocols