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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10529

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Chromogranin A Polyc (CL488-10529)

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Chromogranin A Polyc (CL488-10529)

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 488-conjugated Chromogranin A Polyc (CL488-10529)

    FC experiment of Neuro-2a using CL488-10529

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Chromogranin A (CL488-10529) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse pancreas tissue
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10529 targets Chromogranin A in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin A fusion protein Ag0807 相同性解析による交差性が予測される生物種
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
GenBank accession numberBC006459
Gene symbol Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2883077
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Chromogranin A antibody CL488-10529Download protocol
Standard Protocols
Click here to view our Standard Protocols