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Target

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Species Reactivity

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Conjugate

CoraLite® Plus 647-conjugated Chromogranin A Monoclonal antibody

Chromogranin A Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3H11C11

Cat no : CL647-60135

Synonyms

CHGA, CGA, EA-92, Chromogranin-A, AL-11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 647-conjugated Chromogranin A Monoc (CL647-60135)

    IF Staining of PC-12 using CL647-60135

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 647 Chromogranin A antibody (CL647-60135, Clone: 3H11C11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 647-conjugated Chromogranin A Monoc (CL647-60135)

    IF Staining of SH-SY5Y using CL647-60135

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 647 Chromogranin A antibody (CL647-60135, Clone: 3H11C11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 647-conjugated Chromogranin A Monoc (CL647-60135)

    FC experiment of Neuro-2a using CL647-60135

    1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Chromogranin A (CL647-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 647-conjugated Chromogranin A Monoc (CL647-60135)

    FC experiment of SH-SY5Y using CL647-60135

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Chromogranin A (CL647-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inPC-12 cells, SH-SY5Y cells
Positive FC (Intra) detected inNeuro-2a cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60135 targets Chromogranin A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Chromogranin A fusion protein Ag0807 相同性解析による交差性が予測される生物種
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
GenBank accession numberBC006459
Gene symbol Chromogranin A
Gene ID (NCBI) 1113
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Chromogranin A antibody CL647-60135Download protocol
FC protocol for CL Plus 647 Chromogranin A antibody CL647-60135Download protocol
Standard Protocols
Click here to view our Standard Protocols