CoraLite®594-conjugated Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (1)

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E9A7

Cat no : CL594-67288

Synonyms

Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, Collagen Type I, collagen, type I, alpha 1, OI4


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Tested Applications

Positive IF-P detected inhuman colon cancer tissue, human breast cancer tissue, mouse skin tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-67288 targets Collagen Type I in IF-P applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 1
Calculated molecular weight139 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_000088
Gene symbol COL1A1
Gene ID (NCBI) 1277
RRIDAB_2920107
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.

Protocols

Product Specific Protocols
IF protocol for CL594 Collagen Type I antibody CL594-67288Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Biol Sci

Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma

Authors - Jingwei Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Areli (Verified Customer) (03-14-2023)

I would use a higher dilution next time as the signal was high.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Y201-MSC cells
CL594 Collagen Type I Antibody Immunofluorescence validation (1:500 dilution) in Y201-MSC cells (Cat no:CL594-67288)