Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis of mouse skin using 22734-1-AP (same clone as 22734-1-PBS)
mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
WB analysis of rat skin using 22734-1-AP (same clone as 22734-1-PBS)
rat skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IP experiment of mouse skin using 22734-1-AP (same clone as 22734-1-PBS)
IP result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:500) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
IF Staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)
Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.
×
Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 22734-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Collagen Type III (N-terminal) Polyclonal antibody, PBS Only

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Cat No. 22734-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22734-1-PBS targets Collagen Type III (N-terminal) in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Collagen Type III (N-terminal) fusion protein Ag18658 相同性解析による交差性が予測される生物種
Full Name	collagen, type III, alpha 1
Calculated molecular weight	1466 aa, 139 kDa
Observed molecular weight	140-180 kDa
GenBank accession number	BC028178
Gene Symbol	COL3A1
Gene ID (NCBI)	1281
RRID	AB_2879158
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02461
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skin using 22734-1-AP (same clone as 22734-1-PBS)

WB analysis of rat skin using 22734-1-AP (same clone as 22734-1-PBS)

IP experiment of mouse skin using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human hepatocirrhosis using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human hepatocirrhosis using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human malignant melanoma using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human malignant melanoma using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse heart using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse heart using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human skin using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human skin using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse kidney using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse kidney using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human skin cancer using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human skin cancer using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human pancreas cancer using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of human pancreas cancer using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse liver using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse liver using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)

IHC staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)

IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)

IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)

IF Staining of human colon using 22734-1-AP (same clone as 22734-1-PBS)

IF Staining of mouse colon using 22734-1-AP (same clone as 22734-1-PBS)

Collagen Type III (N-terminal) Polyclonal antibody, PBS Only

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information