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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated Collagen Type VI Pol (CL488-17023)

    IF Staining of mouse skeletal muscle using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using CoraLite® Plus 488-conjugated Collagen Type VI Pol (CL488-17023)

    IF Staining of human colon using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated COL6A1 Polyclonal antibody

COL6A1 Polyclonal Antibody for IF-P
Cat No. CL488-17023

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue, human colon tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17023 targets COL6A1 in IF-P applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL6A1 fusion protein Ag10288 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_3672666
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12109
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COL6A1 antibody CL488-17023Download protocol
Standard Protocols
Click here to view our Standard Protocols