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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Complement factor B Polyclonal antibody (10170-1-AP)

    WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Complement factor B Polyclonal antibody (10170-1-AP)

    WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Complement factor B Polyclonal antibody (10170-1-AP)

    WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Complement factor B Polyclonal antibody (10170-1-AP)

    WB analysis of human plasma using 10170-1-AP (same clone as 10170-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Complement factor B Polyclonal antibody (10170-1-AP)

    WB analysis of human blood using 10170-1-AP (same clone as 10170-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Polyclonal antibody (10170-1-AP)

    IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Polyclonal antibody (10170-1-AP)

    IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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Complement factor B Polyclonal antibody, PBS Only

Complement factor B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10170-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10170-1-PBS targets Complement factor B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag0226 相同性解析による交差性が予測される生物種
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93-100 kDa, 60-69 kDa
GenBank accession numberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
RRIDAB_2082384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00751
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.