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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

8D2B6

Cat no : CL488-66154

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite® Plus 488-conjugated Complement factor B (CL488-66154)

    IF Staining of human liver using CL488-66154

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Complement factor B antibody (CL488-66154, Clone: 8D2B6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite® Plus 488-conjugated Complement factor B (CL488-66154)

    IF Staining of human liver using CL488-66154

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Complement factor B antibody (CL488-66154, Clone: 8D2B6 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66154 targets Complement factor B in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag19107 相同性解析による交差性が予測される生物種
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC007990
Gene symbol Complement factor B
Gene ID (NCBI) 629
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Complement factor B antibody CL488-66154Download protocol
Standard Protocols
Click here to view our Standard Protocols