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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ChromoTek CoraLite® Plus 647-conjugated TIGIT VHH

CL647-tgt targets TIGIT in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-tgt

Synonyms

TIGIT-Nanobody, TIGIT-single domain antibody (sdAb), DKFZp667A205; FLJ39873; VSIG9; VSTM3

Validation Data Gallery

  • 1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt). Cells were not fixed.

    1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt). Cells were not fixed.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL647-tgt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL647-tgt can also be performed after formaldehyde fixation of the cells.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

  • Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

    Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

  • Live HeLa cells transfected with human TIGIT were probed with human recombinant anti-TIGIT IgG. After formaldehyde fixation, the cells were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta) and with anti-human IgG Nano-Secondary (shuGCL488-2, green). Cell nuclei were stained with DAPI (blue).  Epifluorescence images were acquired with 20x objective and post-processed.

    Live HeLa cells transfected with human TIGIT were probed with human recombinant anti-TIGIT IgG. After formaldehyde fixation, the cells were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta) and with anti-human IgG Nano-Secondary (shuGCL488-2, green). Cell nuclei were stained with DAPI (blue).  Epifluorescence images were acquired with 20x objective and post-processed.

  • Left: 1x10^6 human PBMCs were surface stained with 1ug CoraLite® Plus 647 conjugated TIGIT VHH (CL647-tgt) and CL405 CD3 (CL405-65151). Right: 1x10^6 human PBMCs were surface stained with 1ug CoraLite® Plus 647 conjugated TIGIT VHH (CL647-tgt) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

    Left: 1x10^6 human PBMCs were surface stained with 1ug CoraLite® Plus 647 conjugated TIGIT VHH (CL647-tgt) and CL405 CD3 (CL405-65151). Right: 1x10^6 human PBMCs were surface stained with 1ug CoraLite® Plus 647 conjugated TIGIT VHH (CL647-tgt) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Product Information

CL647-tgt targets TIGIT in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 647 Fluorescent Dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.346 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
RRIDAB_3101926
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C; Shipped at ambient temperature.
BackgroundTIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).