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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cortactin Recombinant antibody (84569-1-RR)

    WB analysis using 84569-1-RR (same clone as 84569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84569-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01412-2

    Sandwich ELISA standard curve of MP01412-2

    Sandwich ELISA standard curve of MP01412-2, Human Cortactin Recombinant Matched Antibody Pair - PBS only. 84569-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1940. 84569-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01412-2

    Sample test of MP01412-2

    The mean Cortactin concentration was determined to be 848.3 ng/mL in A549 cell extract based on a 2.3 mg/mL extract load, 581.1 ng/mL in HEK-293 cell extract based on a 3.3 mg/mL extract load and 493.0 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01412-2

    Cytometric bead array standard curve of MP01412-2

    Cytometric bead array standard curve of MP01412-2, Cortactin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84569-2-PBS. Detection antibody: 84569-1-PBS. Standard: Ag1940. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84569-1-RR

    Affinity and Kinetic Characterization of 84569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

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Cortactin Recombinant antibody, PBS Only (Detector)

Cortactin Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84569-1-PBS
Clone No.241987A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CTTN, SRC8, EMS1, 241987A12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84569-1-PBS targets Cortactin as part of a matched antibody pair:

MP01412-2: 84569-2-PBS capture and 84569-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cortactin fusion protein Ag1940 相同性解析による交差性が予測される生物種
Full Name cortactin
Calculated molecular weight 57 kDa
Observed molecular weight70-85 kDa
GenBank accession numberBC008799
Gene Symbol Cortactin
Gene ID (NCBI) 2017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14247
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).