• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cortactin Recombinant antibody

Cortactin Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241987A12

Cat no : 84569-1-RR

Synonyms

CTTN, SRC8, EMS1, 241987A12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cortactin Recombinant antibody (84569-1-RR)

    WB analysis using 84569-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84569-1-RR

    Affinity and Kinetic Characterization of 84569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84569-1-RR targets Cortactin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cortactin fusion protein Ag1940 相同性解析による交差性が予測される生物種
Full Name cortactin
Calculated molecular weight 57 kDa
Observed molecular weight70-85 kDa
GenBank accession numberBC008799
Gene symbol Cortactin
Gene ID (NCBI) 2017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

Protocols

Product Specific Protocols
WB protocol for Cortactin antibody 84569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols