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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CstF-64 Polyclonal antibody

CstF-64 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26825

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CstF-64 Polyclonal a (CL488-26825)

    IF Staining of HepG2 using CL488-26825

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CstF-64 antibody (CL488-26825) at dilution of 1:200, CL594-phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26825 targets CstF-64 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CstF-64 fusion protein Ag25254 相同性解析による交差性が予測される生物種
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Calculated molecular weight 61 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC017712
Gene symbol CstF-64
Gene ID (NCBI) 1478
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CstF-64 antibody CL488-26825Download protocol
Standard Protocols
Click here to view our Standard Protocols