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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin A2 Recombinant antibody (82148-1-RR)

    WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82148-1-RR (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Cyclin A2 Recombinant antibody (82148-1-RR)

    IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IP result of anti-Cyclin A2 (IP:82148-1-RR, 4ug; Detection:82148-1-RR 1:3000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using Cyclin A2 Recombinant antibody (82148-1-RR)

    IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cyclin A2 Recombinant antibody (82148-1-RR)

    IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclin A2 Recombinant antibody (82148-1-RR)

    IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin A2 antibody (82148-1-RR, Clone: 1C23 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82148-1-PBS

    Affinity and Kinetic Characterization of 82148-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82148-1-RR against Human Cyclin A2 were performed. The affinity constant is below 1 pM.

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Cyclin A2 Recombinant antibody, PBS Only

Cyclin A2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82148-1-PBS
Clone No.1C23

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CCNA2, Cyclin-A, Cyclin A, CCNA, CCN1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82148-1-PBS targets Cyclin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC104783
Gene Symbol Cyclin A2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20248
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.