Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82148-1-RR targets Cyclin A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin A2 |
| Calculated molecular weight | 432 aa, 49 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC104783 |
| Gene Symbol | Cyclin A2 |
| Gene ID (NCBI) | 890 |
| RRID | AB_3086468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20248 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin A2 antibody 82148-1-RR | Download protocol |
| IHC protocol for Cyclin A2 antibody 82148-1-RR | Download protocol |
| IF protocol for Cyclin A2 antibody 82148-1-RR | Download protocol |
| IP protocol for Cyclin A2 antibody 82148-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |