CoraLite® Plus 488-conjugated Cyclin A2 Recombinant antibody
Cyclin A2 Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C23
Cat no : CL488-82148
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82148 targets Cyclin A2 in IF/ICC applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin A2 |
| Calculated molecular weight | 432 aa, 49 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC104783 |
| Gene symbol | Cyclin A2 |
| Gene ID (NCBI) | 890 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Cyclin A2 antibody CL488-82148 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
