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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28603

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal (CL488-28603)

    IF Staining of HT-29 using CL488-28603

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal (CL488-28603)

    IF Staining of HeLa using CL488-28603

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHT-29 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28603 targets Cyclin B1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin B1 fusion protein Ag29426 相同性解析による交差性が予測される生物種
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006510
Gene symbol Cyclin B1
Gene ID (NCBI) 891
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin B1 antibody CL488-28603Download protocol
Standard Protocols
Click here to view our Standard Protocols