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  • Featured Product
  • KD/KO Validated

Cyclin B2 Monoclonal antibody, PBS Only

Cyclin B2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G8B7

Cat no : 67726-1-PBS

Synonyms

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    WB analysis using 67726-1-Ig (same clone as 67726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    WB analysis of HeLa using 67726-1-Ig (same clone as 67726-1-PBS)

    WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of PC-12 using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67726-1-PBS targets Cyclin B2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329 相同性解析による交差性が予測される生物種
Full Name cyclin B2
Calculated molecular weight 398 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC105086
Gene symbol Cyclin B2
Gene ID (NCBI) 9133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.