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  • Featured Product
  • KD/KO Validated

Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G8B7

Cat no : 67726-1-Ig

Synonyms

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    WB analysis using 67726-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    WB analysis of HeLa using 67726-1-Ig

    WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IHC staining of human breast cancer using 67726-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IHC staining of human breast cancer using 67726-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of mouse testis using 67726-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of mouse testis using 67726-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Cyclin B2 Monoclonal antibody (67726-1-Ig)

    IF Staining of PC-12 using 67726-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, K-562 cells, Jurkat cells, HEK-293 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67726-1-Ig targets Cyclin B2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329 相同性解析による交差性が予測される生物種
Full Name cyclin B2
Calculated molecular weight 398 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC105086
Gene symbol Cyclin B2
Gene ID (NCBI) 9133
RRIDAB_2882912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

Protocols

Product Specific Protocols
WB protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IHC protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IF protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols