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CoraLite® Plus 488-conjugated Cyclin B2 Monoclonal antibody
Cyclin B2 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G8B7
Cat no : CL488-67726
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67726 targets Cyclin B2 in IF-P applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cyclin B2 fusion protein Ag16329 相同性解析による交差性が予測される生物種 |
Full Name | cyclin B2 |
Calculated molecular weight | 398 aa, 45 kDa |
GenBank accession number | BC105086 |
Gene symbol | Cyclin B2 |
Gene ID (NCBI) | 9133 |
RRID | AB_2919528 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Cyclin B2 antibody CL488-67726 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |