Cyclin D1 Recombinant antibody, PBS Only (Detector)
Cyclin D1 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
6B10
Cat no : 82681-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
82681-1-PBS targets Cyclin D1 as part of a matched antibody pair:
MP80003-1: 68790-1-PBS capture and 82681-1-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cyclin D1 fusion protein Ag25384 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin D1 |
| Calculated molecular weight | 295 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000076 |
| Gene symbol | Cyclin D1 |
| Gene ID (NCBI) | 595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
