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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin D1 Recombinant antibody

Cyclin D1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6B10

Cat no : 82681-1-RR

Synonyms

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin D1 Recombinant antibody (82681-1-RR)

    WB analysis using 82681-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82681-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cyclin D1 Recombinant antibody (82681-1-RR)

    IHC staining of human oesophagus cancer using 82681-1-RR

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cyclin D1 Recombinant antibody (82681-1-RR)

    IF Staining of MCF-7 using 82681-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (82681-1-RR, Clone: 6B10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82681-1-RR

    Affinity and Kinetic Characterization of 82681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82681-1-RR against Human Cyclin D1 were performed. The affinity constant is 0.153 nM.

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Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HCT 116 cells, SW 1990 cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82681-1-RR targets Cyclin D1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin D1 fusion protein Ag25384 相同性解析による交差性が予測される生物種
Full Name cyclin D1
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000076
Gene symbol Cyclin D1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Protocols

Product Specific Protocols
WB protocol for Cyclin D1 antibody 82681-1-RRDownload protocol
IHC protocol for Cyclin D1 antibody 82681-1-RRDownload protocol
IF protocol for Cyclin D1 antibody 82681-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols