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Cardinal Red™-conjugated Cyclin D1 Monoclonal antibody
Cyclin D1 Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, FC (Intra)
Conjugate
Cardinal Red™ Fluorescent Dye
CloneNo.
2G3G5
Cat no : CR-60186
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CR-60186 targets Cyclin D1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cyclin D1 fusion protein Ag0689 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin D1 |
| Calculated molecular weight | 295 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000076 |
| Gene symbol | Cyclin D1 |
| Gene ID (NCBI) | 595 |
| Conjugate | Cardinal Red™ Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cardinal Red™ Cyclin D1 antibody CR-60186 | Download protocol |
| FC protocol for Cardinal Red™ Cyclin D1 antibody CR-60186 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
