- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated Cyclophilin B Monoclonal antibody
Cyclophilin B Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
8H4F1
Cat no : CL488-66047
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66047 targets Cyclophilin B in WB applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cyclophilin B fusion protein Ag17957 相同性解析による交差性が予測される生物種 |
Full Name | peptidylprolyl isomerase B (cyclophilin B) |
Calculated molecular weight | 216 aa, 24 kDa |
Observed molecular weight | 19-24 kDa |
GenBank accession number | BC032138 |
Gene symbol | Cyclophilin B |
Gene ID (NCBI) | 5479 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CL Plus 488 Cyclophilin B antibody CL488-66047 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |