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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cystatin C Recombinant antibody, PBS Only

Cystatin C Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I20

Cat no : 82441-1-PBS

Synonyms

CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cystatin C Recombinant antibody (82441-1-RR)

    WB analysis using 82441-1-RR (same clone as 82441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Cystatin C Recombinant antibody (82441-1-RR)

    WB analysis of HepG2 using 82441-1-RR (same clone as 82441-1-PBS)

    WB result of Cystatin C antibody (82441-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cystatin C Recombinant antibody (82441-1-RR)

    IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cystatin C Recombinant antibody (82441-1-RR)

    IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82441-1-PBS

    Affinity and Kinetic Characterization of 82441-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82441-1-RR against Human Cystatin C were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82441-1-PBS targets Cystatin C in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cystatin C fusion protein Ag2890 相同性解析による交差性が予測される生物種
Full Name cystatin C
Calculated molecular weight 146 aa, 16 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC013083
Gene symbol Cystatin C
Gene ID (NCBI) 1471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.