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  • Featured Product
  • KD/KO Validated

Cystatin C Recombinant antibody

Cystatin C Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1I20

Cat no : 82441-1-RR

Synonyms

CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cystatin C Recombinant antibody (82441-1-RR)

    WB analysis using 82441-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cystatin C Recombinant antibody (82441-1-RR)

    WB analysis of HepG2 using 82441-1-RR

    WB result of Cystatin C antibody (82441-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cystatin C Recombinant antibody (82441-1-RR)

    IHC staining of human kidney using 82441-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cystatin C Recombinant antibody (82441-1-RR)

    IHC staining of human kidney using 82441-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82441-1-RR

    Affinity and Kinetic Characterization of 82441-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82441-1-RR against Human Cystatin C were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, human milk, human urine
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82441-1-RR targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cystatin C fusion protein Ag2890 相同性解析による交差性が予測される生物種
Full Name cystatin C
Calculated molecular weight 146 aa, 16 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC013083
Gene symbol Cystatin C
Gene ID (NCBI) 1471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

Protocols

Product Specific Protocols
WB protocol for Cystatin C antibody 82441-1-RRDownload protocol
IHC protocol for Cystatin C antibody 82441-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols