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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cystatin C Polyclonal antibody

Cystatin C Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12245

Synonyms

CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CoraLite® Plus 488-conjugated Cystatin C Polyclona (CL488-12245)

    IF Staining of mouse kidney using CL488-12245

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CoraLite® Plus 488-conjugated Cystatin C Polyclona (CL488-12245)

    IF Staining of mouse kidney using CL488-12245

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cystatin C Polyclona (CL488-12245)

    IF Staining of HeLa using CL488-12245

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12245 targets Cystatin C in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cystatin C fusion protein Ag2890 相同性解析による交差性が予測される生物種
Full Name cystatin C
Calculated molecular weight 146 aa, 16 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC013083
Gene symbol Cystatin C
Gene ID (NCBI) 1471
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin C antibody CL488-12245Download protocol
Standard Protocols
Click here to view our Standard Protocols