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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytochrome c Recombinant antibody, PBS Only

Cytochrome c Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-PBS

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Validation Data Gallery

Applications:
  • WB analysis using 83276-1-RR (same clone as 83276-1-PBS)

    WB analysis using 83276-1-RR (same clone as 83276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)

    FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 83276-1-PBS is Cytochrome c Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455 相同性解析による交差性が予測される生物種
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
GenBank accession numberBC009578
Gene symbol CYCS
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.