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Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-RR

Synonyms

CYCS, CYC, 230503E10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytochrome c Recombinant antibody (83276-1-RR)

    WB analysis using 83276-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cytochrome c Recombinant antibody (83276-1-RR)

    WB analysis of HepG2 using 83276-1-RR

    WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytochrome c Recombinant antibody (83276-1-RR)

    IF Staining of HepG2 using 83276-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using Cytochrome c Recombinant antibody (83276-1-RR)

    FC experiment of HeLa using 83276-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83276-1-RR

    Affinity and Kinetic Characterization of 83276-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83276-1-RR against Human Cytochrome c were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83276-1-RR is Cytochrome c Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455 相同性解析による交差性が予測される生物種
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene symbol Cytochrome c
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 83276-1-RRDownload protocol
IF protocol for Cytochrome c antibody 83276-1-RRDownload protocol
FC protocol for Cytochrome c antibody 83276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols