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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-RR

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Validation Data Gallery

Applications:
  • WB analysis using 83276-1-RR

    WB analysis using 83276-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83276-1-RR

    FC experiment of HeLa using 83276-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, mouse kidney tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 83276-1-RR is Cytochrome c Fusion Protein expressed in E. coli.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455 相同性解析による交差性が予測される生物種
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene symbol CYCS
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 83276-1-RRDownload protocol
FC protocol for Cytochrome c antibody 83276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols