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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230503E10

Cat no : CL488-83276

Synonyms

CYCS, CYC, 230503E10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated Cytochrome c Recombi (CL488-83276)

    IF Staining of MCF-7 using CL488-83276

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Cytochrome c antibody (CL488-83276, Clone: 230503E10 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83276 targets Cytochrome c in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455 相同性解析による交差性が予測される生物種
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene symbol Cytochrome c
Gene ID (NCBI) 54205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytochrome c antibody CL488-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols