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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytoglobin Monoclonal antibody, PBS Only

Cytoglobin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C7E11

Cat no : 60228-1-PBS

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of pig heart using 60228-1-Ig (same clone as 60228-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of pig heart using 60228-1-Ig (same clone as 60228-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of rat heart using 60228-1-Ig (same clone as 60228-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of Transfected HEK-293 using 60228-1-Ig (same clone as 60228-1-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human bladder using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human bladder using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60228-1-PBS targets Cytoglobin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161 相同性解析による交差性が予測される生物種
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC029798
Gene symbol CYGB
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.