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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4C7E11

Cat no : 60228-1-Ig

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of human heart using 60228-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis using 60228-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of pig heart using 60228-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of pig heart using 60228-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of rat heart using 60228-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    WB analysis of Transfected HEK-293 using 60228-1-Ig

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bladder tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human bladder using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human bladder using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human liver using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human heart using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IHC staining of human heart using 60228-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IF Staining of HeLa using 60228-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytoglobin Monoclonal antibody (60228-1-Ig)

    IF Staining of HeLa using 60228-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHuman heart, pig heart tissue, human heart tissue, Transfected HEK-293 cells, rabbit heart tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60228-1-Ig targets Cytoglobin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161 相同性解析による交差性が予測される生物種
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC029798
Gene symbol Cytoglobin
Gene ID (NCBI) 114757
RRIDAB_11182383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Protocols

Product Specific Protocols
WB protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IHC protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IF protocol for Cytoglobin antibody 60228-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells.

Authors - Shazhou Ye
View Article
humanWB,IF

Cell Death Dis

ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma.

Authors - Peng Zhou
View Article