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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C7E11

Cat no : CL594-60228

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cytoglobin Monoclonal anti (CL594-60228)

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cytoglobin Monoclonal anti (CL594-60228)

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cytoglobin Monoclonal anti (CL594-60228)

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cytoglobin Monoclonal anti (CL594-60228)

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60228 targets Cytoglobin in applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161 相同性解析による交差性が予測される生物種
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC029798
Gene symbol Cytoglobin
Gene ID (NCBI) 114757
RRIDAB_2883446
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Protocols

Product Specific Protocols
IF protocol for CL594 Cytoglobin antibody CL594-60228Download protocol
Standard Protocols
Click here to view our Standard Protocols