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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555-conjugated Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-18343

Synonyms

KRT10, K10, Cytokeratin-10, Cytokeratin,Keratin, CK-10

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 555-conjugated Cytokeratin 10 Polyc (CL555-18343)

    IF Staining of A431 using CL555-18343

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 555 Cytokeratin 10 antibody (CL555-18343) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 555-conjugated Cytokeratin 10 Polyc (CL555-18343)

    FC experiment of A431 using CL555-18343

    1X10^6 A431 cells were intracellularly stained with 0.5 ug CoraLite® Plus 555 Anti-Human Cytokeratin 10 (CL555-18343) (red), or 0.5 ug CL555 Rabbit Isotype Control Antibody (CL555-30000)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-18343 targets Cytokeratin 10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 10 fusion protein Ag13136 相同性解析による交差性が予測される生物種
Full Name keratin 10
Calculated molecular weight 584 aa, 59 kDa
GenBank accession numberBC034697
Gene symbol Cytokeratin 10
Gene ID (NCBI) 3858
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Protocols

Product Specific Protocols
IF protocol for CL Plus 555 Cytokeratin 10 antibody CL555-18343Download protocol
FC protocol for CL Plus 555 Cytokeratin 10 antibody CL555-18343Download protocol
Standard Protocols
Click here to view our Standard Protocols