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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using CoraLite®594-conjugated Cytokeratin 13 Monoclonal (CL594-66684)

    IF Staining of human cervical cancer using CL594-66684

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 Cytokeratin 13 antibody (CL594-66684, Clone: 1E10G2 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using CoraLite®594-conjugated Cytokeratin 13 Monoclonal (CL594-66684)

    IF Staining of human cervical cancer using CL594-66684

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 Cytokeratin 13 antibody (CL594-66684, Clone: 1E10G2 ) at dilution of 1:100.

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  • KD/KO Validated

CoraLite®594-conjugated Cytokeratin 13 Monoclonal antibody

Cytokeratin 13 Monoclonal Antibody for IF-P
Cat No. CL594-66684
Clone No.1E10G2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF-P

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman cervical cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66684 targets Cytokeratin 13 in IF-P applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2920022
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13646
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia.

Protocols

Product Specific Protocols
IF protocol for CL594 Cytokeratin 13 antibody CL594-66684Download protocol
Standard Protocols
Click here to view our Standard Protocols