CoraLite® Plus 488-conjugated Cytokeratin 15 Monoclonal antibody
Cytokeratin 15 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6B4F8
Cat no : CL488-60247
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-60247 targets Cytokeratin 15 in IF/ICC applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cytokeratin 15 fusion protein Ag0185 相同性解析による交差性が予測される生物種 |
Full Name | keratin 15 |
Calculated molecular weight | 49 kDa |
Observed molecular weight | 49 kDa |
GenBank accession number | BC002641 |
Gene symbol | KRT15 |
Gene ID (NCBI) | 3866 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Cytokeratin 15 antibody CL488-60247 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |