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  • Featured Product
  • KD/KO Validated

Cytokeratin 16 Monoclonal antibody, PBS Only

Cytokeratin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4D8

Cat no : 66802-1-PBS

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    WB analysis of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human milk using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    WB analysis of human milk using 66802-1-Ig (same clone as 66802-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    WB analysis of A549 using 66802-1-Ig (same clone as 66802-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human skin cancer using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human skin cancer using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human cervical cancer using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IHC staining of human cervical cancer using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 16 Monoclonal antibody (66802-1-Ig)

    IF Staining of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (66802-1-Ig, Clone: 2H4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66802-1-PBS targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag11240 相同性解析による交差性が予測される生物種
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC039169
Gene symbol Cytokeratin 16
Gene ID (NCBI) 3868
RRIDAB_2882145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.