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- KD/KO Validated
Cytokeratin 16 Monoclonal antibody
Cytokeratin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2H4D8
Cat no : 66802-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, human milk, A549 cells |
| Positive IHC detected in | human tonsillitis tissue, human cervical cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
66802-1-Ig targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 16 fusion protein Ag11240 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 16 |
| Calculated molecular weight | 473 aa, 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC039169 |
| Gene symbol | Cytokeratin 16 |
| Gene ID (NCBI) | 3868 |
| RRID | AB_2882145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| IF protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| FC protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.
| ||
Nat Commun Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer | ||
Genomics Identification of prognostic biomarkers for cholangiocarcinoma by combined analysis of molecular characteristics of clinical MVI subtypes and molecular subtypes
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