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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 16 Monoclonal antibody

Cytokeratin 16 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H4D8

Cat no : CL488-66802

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Cytokeratin 16 Monoc (CL488-66802)

    WB analysis using CL488-66802

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66802 (Cytokeratin 16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66802 targets Cytokeratin 16 in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag11240 相同性解析による交差性が予測される生物種
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC039169
Gene symbol Cytokeratin 16
Gene ID (NCBI) 3868
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Cytokeratin 16 antibody CL488-66802Download protocol
Standard Protocols
Click here to view our Standard Protocols