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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 18 Polyclonal antibody, PBS Only

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10830-1-PBS

Synonyms

KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    WB analysis using 10830-1-AP (same clone as 10830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IP experiment of A431 using 10830-1-AP (same clone as 10830-1-PBS)

    IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IF Staining of HepG2 using 10830-1-AP (same clone as 10830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    IF Staining of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cytokeratin 18 Polyclonal antibody (10830-1-AP)

    FC experiment of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10830-1-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260 相同性解析による交差性が予測される生物種
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2133164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.