Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10830-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis using 10830-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10830-1-AP
IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.
IHC staining of human breast cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).
IHC staining of human breast cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).
IHC staining of rat liver using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary tumor using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human endometrial cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human endometrial cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human renal cell carcinoma using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).
IHC staining of human kidney using 10830-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).
IF Staining of HeLa using 10830-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10830-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of HeLa using 10830-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10830-1-AP

Publications(116)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (116)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, K-562 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human breast cancer tissue, human appendicitis tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human prostate hyperplasia tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsillitis tissue, mouse liver tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 37 publications below
IHC	See 25 publications below
IF	See 56 publications below
FC	See 2 publications below

Product Information

10830-1-AP targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, bovine, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 18 fusion protein Ag1260 相同性解析による交差性が予測される生物種
Full Name	keratin 18
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC000180
Gene Symbol	Cytokeratin 18
Gene ID (NCBI)	3875
RRID	AB_2133164
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05783
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 10830-1-AP	Download protocol
IHC protocol for Cytokeratin 18 antibody 10830-1-AP	Download protocol
IF protocol for Cytokeratin 18 antibody 10830-1-AP	Download protocol
IP protocol for Cytokeratin 18 antibody 10830-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. Authors - Brian Lin View Article
mouse	IF	Hepatology Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development. Authors - Bing Han View Article
rat	IHC	J Control Release Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration Authors - Yue Tang View Article
rat	IF	Acta Biomater A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. Authors - Yingzheng Zhao View Article
mouse	IHC,IF	Proc Natl Acad Sci U S A Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice. Authors - Wada-Hiraike Osamu O View Article
mouse	IF	Proc Natl Acad Sci U S A Basal epithelial stem cells are efficient targets for prostate cancer initiation. Authors - Lawson Devon A DA View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10830-1-AP

WB analysis using 10830-1-AP

IP experiment of A431 using 10830-1-AP

IHC staining of human breast cancer using 10830-1-AP

IHC staining of human breast cancer using 10830-1-AP

IHC staining of rat liver using 10830-1-AP

IHC staining of rat liver using 10830-1-AP

IHC staining of mouse liver using 10830-1-AP

IHC staining of mouse liver using 10830-1-AP

IHC staining of human renal cell carcinoma using 10830-1-AP

IHC staining of human renal cell carcinoma using 10830-1-AP

IHC staining of human appendicitis using 10830-1-AP

IHC staining of human colon cancer using 10830-1-AP

IHC staining of human colon cancer using 10830-1-AP

IHC staining of human lung cancer using 10830-1-AP

IHC staining of human lung cancer using 10830-1-AP

IHC staining of human tonsillitis using 10830-1-AP

IHC staining of human tonsillitis using 10830-1-AP

IHC staining of human appendicitis using 10830-1-AP

IHC staining of human prostate hyperplasia using 10830-1-AP

IHC staining of human prostate hyperplasia using 10830-1-AP

IHC staining of human ovary tumor using 10830-1-AP

IHC staining of human ovary tumor using 10830-1-AP

IHC staining of human stomach cancer using 10830-1-AP

IHC staining of human stomach cancer using 10830-1-AP

IHC staining of human endometrial cancer using 10830-1-AP

IHC staining of human endometrial cancer using 10830-1-AP

IHC staining of human pancreas cancer using 10830-1-AP

IHC staining of human pancreas cancer using 10830-1-AP

IHC staining of human liver cancer using 10830-1-AP

IHC staining of human liver cancer using 10830-1-AP

IHC staining of human renal cell carcinoma using 10830-1-AP

IHC staining of human renal cell carcinoma using 10830-1-AP

IHC staining of human lung cancer using 10830-1-AP

IHC staining of human lung cancer using 10830-1-AP

IHC staining of human kidney using 10830-1-AP

IHC staining of human kidney using 10830-1-AP

IF Staining of HeLa using 10830-1-AP

IF Staining of HepG2 using 10830-1-AP

FC experiment of HeLa using 10830-1-AP

Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.

Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.

Basal epithelial stem cells are efficient targets for prostate cancer initiation.