CoraLite® Plus 488-conjugated Cytokeratin 20 Monoclonal antibody
Cytokeratin 20 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4D10A4
Cat no : CL488-60183
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human colon tissue |
| Positive IF/ICC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60183 targets Cytokeratin 20 in IF/ICC, IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 20 fusion protein Ag11336 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 20 |
| Calculated molecular weight | 424 aa, 48 kDa |
| GenBank accession number | BC031559 |
| Gene symbol | Cytokeratin 20 |
| Gene ID (NCBI) | 54474 |
| RRID | AB_2919234 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-60183 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
