Validation Data Gallery
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Tested Applications
| Positive WB detected in | HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cervical cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1150-1:4600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 5/6 fusion protein Ag11288 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 6B |
| Calculated molecular weight | 564 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC034535 |
| Gene Symbol | Cytokeratin 6B |
| Gene ID (NCBI) | 3854 |
| RRID | AB_2935375 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P04259 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| IF protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |