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Cytokeratin 5/6 Monoclonal antibody

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 68295-1-Ig
Clone No.1F9E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman cervical cancer tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1150-1:4600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 5/6 fusion protein Ag11288 相同性解析による交差性が予測される生物種
Full Name keratin 6B
Calculated molecular weight 564 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
RRIDAB_2935375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
IHC protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
IF protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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