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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 5/6 Monoclonal antibody, PBS Only

Cytokeratin 5/6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9E1

Cat no : 68295-1-PBS

Synonyms

CK 6B, CK5/6, CK6B, Cytokeratin 6B, K6B, keratin 5, keratin 6B, KRT6B, KRTL1, PC2, Type II keratin Kb10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68295-1-PBS targets Cytokeratin 5/6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 5/6 fusion protein Ag11288 相同性解析による交差性が予測される生物種
Full Name keratin 6B
Calculated molecular weight 564 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC034535
Gene symbol KRT6B
Gene ID (NCBI) 3854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.