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  • Featured Product
  • KD/KO Validated

Biotin-conjugated Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

2E1G5

Cat no : Biotin-66483

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biotin-conjugated Cytokeratin 7 Monoclonal antibod (Biotin-66483)

    IHC staining of human lung cancer using Biotin-66483

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Biotin-66483 (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biotin-conjugated Cytokeratin 7 Monoclonal antibod (Biotin-66483)

    IHC staining of human lung cancer using Biotin-66483

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Biotin-66483 (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biotin-conjugated Cytokeratin 7 Monoclonal antibod (Biotin-66483)

    IHC staining of human lung cancer using Biotin-66483

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Biotin-66483 (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biotin-conjugated Cytokeratin 7 Monoclonal antibod (Biotin-66483)

    IHC staining of human lung cancer using Biotin-66483

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Biotin-66483 (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66483 targets Cytokeratin 7 in IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag7895 相同性解析による交差性が予測される生物種
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
GenBank accession numberBC002700
Gene symbol Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2883073
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Protocols

Product Specific Protocols
IHC protocol for Biotin Cytokeratin 7 antibody Biotin-66483Download protocol
Standard Protocols
Click here to view our Standard Protocols