- Featured Product
- KD/KO Validated
DACH1 Monoclonal antibody, PBS Only
DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3B6D2
Cat no : 60082-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
60082-1-PBS targets DACH1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | DACH1 fusion protein Ag4474 相同性解析による交差性が予測される生物種 |
Full Name | dachshund homolog 1 (Drosophila) |
Calculated molecular weight | 79 kDa |
Observed molecular weight | 97-110 kDa |
GenBank accession number | BC021219 |
Gene symbol | DACH1 |
Gene ID (NCBI) | 1602 |
RRID | AB_2089711 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.