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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    WB analysis of HEK-293 using 60082-1-Ig

    WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells.

  • Western Blot (WB) analysis of MCF-7 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    WB analysis of MCF-7 using 60082-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    WB analysis of ROS1728 using 60082-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    WB analysis of C6 using 60082-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    WB analysis of L02 using 60082-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of mouse brain using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of mouse brain using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of human endometrial cancer using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of human endometrial cancer using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of human prostate cancer using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IHC staining of human prostate cancer using 60082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IF Staining of mouse brain using 60082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IF Staining of mouse embryo using 60082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using DACH1 Monoclonal antibody (60082-1-Ig)

    IF Staining of mouse embryo using 60082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DACH1 Monoclonal antibody (60082-1-Ig)

    IF Staining of HEK-293 using 60082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60082-1-Ig
Clone No.3B6D2
Publications(4)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

Dachshund-1, DACH, 3B6D2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, C6 cells, L02 cells, ROS1728 cells, HEK-293 cells
Positive IHC detected inmouse brain tissue, human endometrial cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse embryo tissue, mouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60082-1-Ig targets DACH1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DACH1 fusion protein Ag4474 相同性解析による交差性が予測される生物種
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
Observed molecular weight 97-110 kDa
GenBank accession numberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2089711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UI36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

Protocols

Product Specific Protocols
WB protocol for DACH1 antibody 60082-1-IgDownload protocol
IHC protocol for DACH1 antibody 60082-1-IgDownload protocol
IF protocol for DACH1 antibody 60082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP,IF

Am J Respir Cell Mol Biol

DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling

Authors - Qian Huang
View Article
humanWB

Cell Biol Int

DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma

Authors - Shengru Liang
View Article
humanWB

Front Oncol

Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas

Authors - Qiang Gu
  • KD Validated
View Article
humanWB

Clin Exp Metastasis

Lung-derived soluble factors support stemness/plasticity and metastatic behaviour of breast cancer cells via the FGF2-DACH1 axis

Authors - Vasudeva Bhat
View Article