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  • Featured Product
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CoraLite® Plus 488-conjugated DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6D2

Cat no : CL488-60082

Synonyms

Dachshund-1, DACH, 3B6D2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated DACH1 Monoclonal ant (CL488-60082)

    IF Staining of mouse brain using CL488-60082

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated DACH1 Monoclonal ant (CL488-60082)

    IF Staining of mouse brain using CL488-60082

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60082 targets DACH1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DACH1 fusion protein Ag4474 相同性解析による交差性が予測される生物種
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
GenBank accession numberBC021219
Gene symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2883099
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110 kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DACH1 antibody CL488-60082Download protocol
Standard Protocols
Click here to view our Standard Protocols